Disposable fluid circuits and methods for cell washing with on-line dilution of cell feed
First Claim
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1. A method for washing biological cells comprising:
- obtaining a separator comprising a relatively rotatable cylindrical housing and an internal member, wherein said cylindrical housing has an interior surface and said internal member has an exterior surface, said surfaces defining a gap therebetween, wherein at least one of said surfaces includes a porous membrane;
drawing biological cells from a container in flow communication with said separator;
diluting said biological cells with a diluent;
introducing said diluted cells and diluent into said gap of said separator;
rotating at least one or both of said housing and said internal member;
separating said cells from said liquid medium;
concentrating said cells;
removing at least some of said concentrated cells from said separator through a first outlet;
removing at least some of said separated liquid medium from said separator through a second outlet;
detecting pressure inside of said separator wherein said pressure is based at least in part on the build-up of cellular material on said membrane; and
adjusting the dilution of said cells if said detected pressure differs from a selected pressure wherein the selected pressure corresponds to a concentration polarization limit.
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Abstract
Systems and methods for the washing and processsing of biological fluid/biological cells are disclosed. The systems and methods prevent inadvertent target cell loss by monitoring pressure and providing for the dilution of the cell feed.
24 Citations
16 Claims
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1. A method for washing biological cells comprising:
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obtaining a separator comprising a relatively rotatable cylindrical housing and an internal member, wherein said cylindrical housing has an interior surface and said internal member has an exterior surface, said surfaces defining a gap therebetween, wherein at least one of said surfaces includes a porous membrane; drawing biological cells from a container in flow communication with said separator; diluting said biological cells with a diluent; introducing said diluted cells and diluent into said gap of said separator; rotating at least one or both of said housing and said internal member; separating said cells from said liquid medium; concentrating said cells; removing at least some of said concentrated cells from said separator through a first outlet; removing at least some of said separated liquid medium from said separator through a second outlet; detecting pressure inside of said separator wherein said pressure is based at least in part on the build-up of cellular material on said membrane; and adjusting the dilution of said cells if said detected pressure differs from a selected pressure wherein the selected pressure corresponds to a concentration polarization limit.
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2. A method for washing biological cells comprising:
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obtaining a separator comprising a cylindrical housing and an internal member, wherein said cylindrical housing has an interior surface and said internal member has an exterior surface including a porous membrane, said surfaces defining a circumferential gap therebetween and about said internal member; drawing biological cells from a container in flow communication with said separator; diluting said biological cells with a diluent; introducing said diluted cells and diluent into said gap of said separator; rotating said internal member; separating said cells from said liquid medium; concentrating said cells; removing at least some of said concentrated cells from said gap through a first outlet; removing at least some of said separated liquid medium from said separator through a second outlet; detecting pressure inside of said separator wherein said pressure is based at least in part on the build-up of cellular material on said membrane; and adjusting the dilution of said cells if said detected pressure differs from a selected pressure. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification