Reduced size optical coupler for fluorescence detection

• US 9,801,578 B2
• Filed: 01/21/2014
• Issued: 10/31/2017
• Est. Priority Date: 01/22/2013
• Status: Active Grant

• Current Assignee: Embecta Corporation
• Original Assignee: Becton, Dickinson & Co
• Inventors: Weber, Aaron, Tracy, David, Salemme, James, Prudden, John
• Primary Examiner(s): Winakur, Eric
• Assistant Examiner(s): Liu, Chu Chuan (JJ)
• Application Number:
  US14/762,447
• Publication Number:
  US 20150351669A1
• Time in Patent Office: 1,379 Days
• CPC Class Codes: A61B 2562/0233 Special features of optical sensors or probes classified in A61B5/00
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: A61B 5/1545 comprising means for indicating vein or arterial entry
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides

Abstract

Optical systems are disclosed for use in identifying an analyte, such as glucose in blood or interstitial fluid (ISF), using a biomaterial, such as glucose binding protein (GBP), that is brought into contact with the analyte. An optical system includes a first filter adapted to reflect light emitted from a light-emitting diode to illuminate a fluorescent body, and further adapted to transmit light emitted from the fluorescent body, and a second filter adapted to separate light transmitted by the first filter into signal band light and reference band light.

Claims

1. An optical system comprising:

a first filter adapted to reflect light emitted from a light-emitting diode in a first frequency band to illuminate a fluorescent body, and further adapted to transmit light emitted from the fluorescent body in a second frequency band;

a second filter adapted to separate light transmitted by the first filter by reflecting a signal band light and transmitting a reference band light; and

a single optical connector comprising at least two integral lenses and an inclined surface upon which said first filter is mounted and said second filter is positioned to direct the reflected signal band light through the inclined surface.

2. The optical system of claim 1, further comprising a photodiode adapted to receive at least one selected from the set consisting of signal band light and reference band light transmitted by the second filter.

3. The optical system of claim 1, wherein the first filter comprises a first glass filter comprising a first dichroic filter coating on a first surface of the first glass filter,

wherein the first dichroic filter is adapted to reflect light emitted by the light-emitting diode and is further adapted to transmit light emitted from the fluorescent body.

4. The optical system of claim 1, wherein the first filter is further adapted to block wavelengths outside of a signal band wavelength range and a reference band wavelength range.

5. The optical system of claim 1, wherein the first filter comprises a glass filter coated by a dichroic filter coating.

6. The optical system of claim 1, wherein the second filter comprises a second glass filter comprising a second dichroic filter coating on a first surface of the second glass filter,

wherein the second dichroic filter is adapted to reflect light of wavelengths associated with a signal band, and is further adapted to transmit light of wavelengths associated with a reference band.

7. The optical system of claim 1, wherein wavelengths associated with the signal band are shorter than wavelengths associated with the reference band.

8. The optical system of claim 1, wherein the second filter comprises a diffraction grating layer adapted to spread light in accordance with wavelength of light.

9. The optical system of claim 1, wherein the first filter and the second filter are formed on opposing sides of a filter element.

10. An optical system comprising:

a first filter adapted to reflect light emitted from a light-emitting diode in a first frequency band to illuminate a fluorescent body, and further adapted to transmit light emitted from the fluorescent body in a second frequency band;

a second filter adapted to separate light transmitted by the first filter by reflecting a first beam of light in a signal band and transmitting a second beam of light in a reference band; and

a single optical connector comprising at least two integral lenses and an inclined surface upon which said first filter is mounted and said second filter is positioned to direct the first beam of light through the inclined surface, wherein the second filter is adapted to separate light transmitted by the first filter substantially evenly into the first beam and the second beam.

11. The optical system of claim 10, further comprising a photodiode adapted to receive at least one selected from the set consisting of the first beam and the second beam.

12. The optical system of claim 11, wherein the photodiode comprises a filter that blocks wavelengths outside of one selected from the set consisting of a signal band wavelength range and a reference band wavelength range.

13. The optical system of claim 12, wherein the filter comprises a filter coating.

14. The optical system of claim 10, wherein the first filter and the second filter are formed into a unitary component.

15. An optical coupler comprising:

a fiber adapted to contact a fluorescent body;

a light-emitting diode;

a first filter adapted to reflect light emitted from the light-emitting diode in a first frequency band to illuminate the fluorescent body through the fiber, and further adapted to transmit light emitted from the fluorescent body in a second frequency band;

a second filter adapted to separate light transmitted by the first filter by reflecting a signal band light and transmitting a reference band light;

a single optical connector comprising at least two integral lenses and an inclined surface upon which said first filter is mounted and said second filter is positioned to direct the reflected signal band light through the inclined surface; and

a photodiode adapted to receive at least one selected from the set consisting of signal band light and reference band light transmitted by the second filter.

16. The optical coupler of claim 15, further comprising:

an light-emitting diode lens adapted to focus emitted from the light-emitting diode toward the first filter;

a fiber lens adapted to focus light toward the fiber, and further adapted to focus light from the fiber; and

a detector lens adapted to focus light onto the photodiode.

17. The optical coupler of claim 16, wherein the light-emitting diode lens and the fiber lens are integrally formed.

18. An optical sensor comprising:

the optical coupler of claim 15; and

a base with a bottom surface and a top surface, wherein the bottom surface is adherable to skin,

wherein the light-emitting diode is disposed on the top surface of the base, and

wherein the photodiode is disposed on the top surface of the base.

19. The optical system of claim 15, wherein the fluorescent body comprises a biomaterial.

20. The optical system of claim 15, wherein the fluorescent body comprises a glucose binding protein.