Bioeffective krill oil compositions

• US 9,816,046 B2
• Filed: 05/08/2017
• Issued: 11/14/2017
• Est. Priority Date: 03/28/2007
• Status: Active Grant

• Current Assignee: Aker BioMarine Antarctic AS (Aker BioMarine ASA)
• Original Assignee: Aker BioMarine Antarctic AS (Aker BioMarine ASA)
• Inventors: Bruheim, Inge, Tilseth, Snorre, Mancinelli, Daniele
• Primary Examiner(s): Cutliff, Yate K
• Application Number:
  US15/589,572
• Publication Number:
  US 20170240842A1
• Time in Patent Office: 190 Days
• Field of Search: None
• CPC Class Codes: A23L 33/115 Fatty acids or derivatives thereof; Fats or oils
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/122 having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
• CPC Class Codes: A61K 31/20 having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
• CPC Class Codes: A61K 31/202 having three or more double bonds, e.g. linolenic eicosanoids, e.g. leukotrienes A61K31/557
• CPC Class Codes: A61K 31/215 of carboxylic acids
• CPC Class Codes: A61K 31/23 of acids having a carboxyl group bound to a chain of seven or more carbon atoms
• CPC Class Codes: A61K 31/235 having an aromatic ring attached to a carboxyl group
• CPC Class Codes: A61K 31/575 substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
• CPC Class Codes: A61K 31/683 Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
• CPC Class Codes: A61K 31/685 one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin
• CPC Class Codes: A61K 35/612 Crustaceans, e.g. crabs, lobsters, shrimps, krill or crayfish; Barnacles
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61K 9/48 Preparations in capsules, e.g. of gelatin, of chocolate A61K9/0004 takes precedence; bite capsules A61K9/0056
• CPC Class Codes: A61K 9/4825 Proteins, e.g. gelatin gelatin capsule shells with substantial amounts of other macromolecular substances A61K9/4816
• CPC Class Codes: A61K 9/4858 Organic compounds
• CPC Class Codes: A61P 3/02 Nutrients, e.g. vitamins, minerals
• CPC Class Codes: C11B 3/006 by extraction

Abstract

This invention discloses new krill oil compositions characterized by having high amounts of phospholipids, astaxanthin esters and/or omega-3 contents. The krill oils are obtained from krill meal using supercritical fluid extraction in a two stage process. Stage 1 removes the neutral lipid by extracting with neat supercritical CO₂ or CO₂ plus approximately 5% of a co-solvent. Stage 2 extracts the actual krill oils by using supercritical CO₂ in combination with approximately 20% ethanol. The krill oil materials obtained are compared with commercially available krill oil and found to be more bioeffective in a number of areas such as anti-inflammation, anti-oxidant effects, improving insulin resistances and improving blood lipid profile.

Claims

1. A method of production of krill oil comprising:

obtaining a krill meal produced by a process comprising treating krill to destroy the activity of lipases and phospholipases naturally present in krill and wherein said krill meal has been stored for period of from 1 to 36 months; and

extracting krill oil from said krill meal that has been stored from 1 to 36 months with a polar solvent to provide a krill oil with greater than 30% phosphatidylcholine w/w of said krill oil and astaxanthin esters.

2. The method of claim 1, wherein said krill oil comprises less than 3% free fatty acids w/w of said krill oil.

3. The method of claim 1, wherein said krill oil comprises less than about 2% lysophosphatidylcholine w/w of said krill oil.

4. The method of claim 1, wherein said process comprising treating krill to destroy the activity of the lipases and phospholipases naturally present in krill comprises grinding said krill prior to destroying the activity of said lipases and phospholipases naturally present in krill.

5. The method of claim 1, wherein said process comprising treating krill to destroy the activity of the lipases and phospholipases naturally present in krill comprises heating said krill.

6. The method of claim 1, wherein said process comprising treating krill to destroy the activity of the lipases and phospholipases naturally present in krill comprises treating said krill with chemicals.

7. The method of claim 1, wherein said process comprising treating krill to destroy the activity of the lipases and phospholipases naturally present in krill comprises a combination of heating said krill and treating said krill with chemicals.

8. The method of claim 1, further comprising encapsulating said krill oil.

9. The method of claim 3, wherein said krill is Euphausia superba.

10. The method of claim 1, wherein said krill oil comprises at least 40% phosphatidylcholine w/w of said krill oil.

11. The method of claim 1, wherein said astaxanthin esters are present in the krill oil at in an amount of at least 100 mg/kg.

12. The method of claim 1, wherein said astaxanthin esters are present in the krill oil at in an amount of at least 200 mg/kg.

13. A method of production of Euphausia superba krill oil comprising:

a) obtaining a Euphausia superba krill meal produced by a process comprising treating Euphausia superba to destroy the activity of lipases and phospholipases naturally present in Euphausia superba and wherein said Euphausia superba krill meal has been stored from 1 to 36 months; and

b) extracting Euphausia superba oil from said krill meal that has been stored from 1 to 36 months with a polar solvent to provide a Euphausia superba krill oil comprising greater than 30% phosphatidylcholine w/w of said Euphausia superba krill oil, less than 3% free fatty acids w/w of said Euphausia superba krill oil, and at least 100 mg/kg astaxanthin esters.

14. The method of claim 13, further comprising encapsulating said Euphausia superba krill oil.

15. The method of claim 13, wherein said krill oil comprises at least 40% phosphatidylcholine w/w of said Euphausia superba krill oil.

16. The method of claim 13, wherein said process comprising treating Euphausia superba to destroy the activity of the lipases and phospholipases naturally present in Euphausia superba comprises grinding said Euphausia superba prior to destroying the activity of said lipases and phospholipases naturally present in Euphausia superba.

17. The method of claim 13, wherein said process comprising treating Euphausia superba to destroy the activity of the lipases and phospholipases naturally present in Euphausia superba comprises heating said Euphausia superba.

18. The method of claim 13, wherein said process comprising treating Euphausia superba to destroy the activity of the lipases and phospholipases naturally present in Euphausia superba comprises treating said Euphausia superba with chemicals.

19. The method of claim 13, wherein said process comprising treating Euphausia superba to destroy the activity of the lipases and phospholipases naturally present in Euphausia superba comprises a combination of heating said Euphausia superba and treating said Euphausia superba with chemicals.