Calibration vial and technique for calibrating a fiber optic oxygen sensing needle

• US 9,915,602 B2
• Filed: 02/11/2015
• Issued: 03/13/2018
• Est. Priority Date: 04/20/2012
• Status: Active Grant

• Current Assignee: MOCON, Inc. (Ametek Incorporated)
• Original Assignee: MOCON, Inc. (Ametek Incorporated)
• Inventors: Eastman, John
• Primary Examiner(s): Raevis, Robert R
• Application Number:
  US14/619,166
• Publication Number:
  US 20150153282A1
• Time in Patent Office: 1,126 Days
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 21/278 Constitution of standards
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 2201/088 Using a sensor fibre

Abstract

A calibration tool and method of using the tool to calibrate a fiber optic needle oxygen sensor. The tool includes at least a vial sealingly covered by a septa and containing a supply of particulate oxygen getter within the chamber of the vial. The vial has an open top and is constructed from an oxygen impermeable material. The septa is resealing, needle-penetrable and oxygen impermeable. The supply of particulate oxygen getter is retained within an oxygen permeable sachet.

Claims

1. A calibration tool for use in obtaining a zero calibration value for a fiber optic needle oxygen sensor, consisting essentially of:

(a) an open top vial constructed from an oxygen impermeable material and defining a chamber,

(b) a resealing, needle-penetrable, oxygen impermeable septa sealingly engaged over the open top of the vial, and

(c) an oxygen permeable sachet containing a mass of particulate oxygen getter retained within the sealed chamber.

2. The calibration tool of claim 1, wherein the vial is a small container.

3. The calibration tool of claim 1, wherein the oxygen getter is iron powder having an average particle size of 1 to 5 mil.

4. The calibration tool of claim 2, wherein the weight/volume ratio of iron/sealed chamber is 1:

10 to 1;

20 mg/cm³.

5. A method of calibrating a fiber optic needle oxygen sensor having an oxygen sensitive photoluminescent probe proximate the tip of the needle, comprising the steps of:

(a) setting the sensor to calibration mode,

(b) obtaining a span calibration value by (i) placing the photoluminescent probe of the sensor in fluid communication with a fluid having a known non-zero partial pressure of oxygen, (ii) taking an oxygen concentration reading with the photoluminescent probe in fluid communication with a fluid having a known non-zero partial pressure of oxygen, and (iii) correlating the oxygen concentration reading with the known non-zero oxygen partial pressure, and

(c) obtaining a zero calibration value by (i) placing the photoluminescent probe of the sensor in fluid communication with the chamber of the calibration tool of claim 2 by inserting the needle through the septa on the calibration tool, (ii) taking an oxygen concentration reading with the photoluminescent probe in fluid communication with the chamber of the calibration tool, and (iii) correlating the oxygen concentration reading with the known near zero oxygen partial pressure in the chamber.

6. The method of claim 5 wherein the span calibration value and the zero calibration value are taken in a predetermined sequence.

7. The method of claim 5 further comprising the step of providing the oxygen sensor with data indicating whether a first reading was a span calibration value or a zero calibration value.

8. The method of claim 5 wherein the span calibration value is obtained by placing the photoluminescent probe in fluid communication with the atmosphere.