Method for collecting functional cells in vivo with high efficiency
First Claim
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1. A method for collecting bone-marrow-derived stem cells from a subject, wherein the method comprises:
- (I) implanting for at least 12 hours a vessel that contains a substance of any of (a) to (j) below, completely under the skin in subcutaneous adipose of the subject, to induce stem cells present in the bone marrow of the subject to migrate to the vessel implanted in subcutaneous tissue, wherein, as a result of the presence of the substance, stem cells present in bone marrow of the subject enter the vessel implanted in subcutaneous adipose;
(a) an HMGB1 protein,(b) a cell that secretes an HMGB1,(c) an HMGB2 protein,(d) a cell that secretes an HMGB2,(e) an HMGB3 protein,(f) a cell that secretes an HMGB3 protein,(g) an S100A8 protein,(h) a cell that secretes an S100A8 protein,(i) an S100A9 protein,and (j) a cell that secretes an S100A9 protein,wherein the vessel is made from a material that is biologically hypoallergenic when implanted into the subject without any of (a) to (j), and wherein the vessel has one closed end to form an area therein to collect the bone-marrow-derived stem cells; and
(II) collecting bone-marrow-derived stem cells from the vessel.
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Abstract
Biologically low invasive vessels are filled with biological factors that have the activity of mobilizing specific functional cells in the body. The vessels are indwelled in the body. After specific functional cells are mobilized into the vessels, the vessels are removed from the body to collect functional cell populations mobilized to the vessels. Alternatively, the cells are directly collected from the vessels indwelled in the body.
30 Citations
27 Claims
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1. A method for collecting bone-marrow-derived stem cells from a subject, wherein the method comprises:
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(I) implanting for at least 12 hours a vessel that contains a substance of any of (a) to (j) below, completely under the skin in subcutaneous adipose of the subject, to induce stem cells present in the bone marrow of the subject to migrate to the vessel implanted in subcutaneous tissue, wherein, as a result of the presence of the substance, stem cells present in bone marrow of the subject enter the vessel implanted in subcutaneous adipose; (a) an HMGB1 protein, (b) a cell that secretes an HMGB1, (c) an HMGB2 protein, (d) a cell that secretes an HMGB2, (e) an HMGB3 protein, (f) a cell that secretes an HMGB3 protein, (g) an S100A8 protein, (h) a cell that secretes an S100A8 protein, (i) an S100A9 protein, and (j) a cell that secretes an S100A9 protein, wherein the vessel is made from a material that is biologically hypoallergenic when implanted into the subject without any of (a) to (j), and wherein the vessel has one closed end to form an area therein to collect the bone-marrow-derived stem cells; and (II) collecting bone-marrow-derived stem cells from the vessel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for collecting bone-marrow-derived stem cells from a subject, wherein the method comprises:
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(I) implanting for at least 12 hours a vessel, that contains a substance of any of (a) to (j) below, completely under the skin in subcutaneous adipose of the subject, to induce stem cells present in the bone marrow of the subject to migrate to the vessel implanted in subcutaneous tissue, wherein, as a result of the presence of the substance, stem cells present in bone marrow of the subject enter the vessel implanted in subcutaneous adipose; (a) an HMGB1 protein, (b) a cell that secretes an HMGB1, (c) an HMGB2 protein, (d) a cell that secretes an HMGB2, (e) an HMGB3 protein, (f) a cell that secretes an HMGB3 protein, (g) an S100A8 protein, (h) a cell that secretes an S100A8 protein, (i) an S100A9 protein, and (j) a cell that secretes an S100A9 protein, wherein the vessel is made from a material that is biologically hypoallergenic when implanted into the subject without any of (a) to (j), and wherein the vessel has one closed end to form an area therein to collect the bone-marrow-derived stem cells; (II) removing the vessel to outside the body from under the skin in subcutaneous adipose of the subject; and (III) collecting bone-marrow-derived stem cells from the vessel. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for collecting bone-marrow-derived stem cells from a subject, wherein the method comprises:
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(I) implanting for at least 12 hours a vessel that contains a substance of any of (a) to (j) below, completely under the skin in subcutaneous adipose of the subject, to induce stem cells present in the bone marrow of the subject to migrate to the vessel implanted in subcutaneous tissue, wherein, as a result of the presence of the substance, stem cells present in bone marrow of the subject enter the vessel implanted in subcutaneous adipose; (a) an HMGB1 protein, (b) a cell that secretes an HMGB1, (c) an HMGB2 protein, (d) a cell that secretes an HMGB2, (e) an HMGB3 protein, (f) a cell that secretes an HMGB3 protein, (g) an S100A8 protein, (h) a cell that secretes an S100A8 protein, (i) an S100A9 protein, and (j) a cell that secretes an S100A9 protein, wherein the vessel is made from a material that is biologically hypoallergenic when implanted into the subject without any of (a) to (j), and wherein the vessel has one closed end to form an area therein to collect the bone-marrow-derived stem cells; and (II) collecting bone-marrow-derived stem cells from the vessel, which is still under the skin in subcutaneous adipose of the subject. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27)
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Specification