Systems and methods for identifying cosmetic agents for hair/scalp care compositions
First Claim
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1. A method for constructing a data architecture for use in identifying connections between perturbagens and genes associated with one or more cosmetic hair biology conditions and preparing a hair/scalp care composition, comprising:
- (a) providing a gene expression profile for a control human fibroblast cell;
(b) generating a gene expression profile for a human fibroblast cell exposed to at least one perturbagen by extracting a biological sample from the exposed cell and subjecting the biological sample to microarray analysis via a microarray scanner;
(c) identifying genes differentially expressed in response to the at least one perturbagen by comparing the gene expression profiles of (a) and (b);
(d) creating an ordered list comprising identifiers representing the differentially expressed genes, wherein the identifiers are ordered according to the differential expression of the genes;
(e) storing the ordered list as a fibroblast instance on at least one computer readable medium; and
(f) constructing a data architecture of stored fibroblast instances by repeating (a) through (e), wherein the at least one perturbagen of step (a) is different for each fibroblast instance;
(g) providing a gene expression profile for a control human keratinocyte cell;
(h) generating a gene expression profile for a human keratinocyte cell exposed to at least one perturbagen by extracting a biological sample from the exposed cell and subjecting the biological sample to microarray analysis via a microarray scanner;
(i) identifying genes differentially expressed in response to the at least one perturbagen by comparing the gene expression profiles of (g) and (h);
(j) creating an ordered list comprising identifiers representing the differentially expressed genes, wherein the identifiers are ordered according to the differential expression of the genes identified in (i);
(k) storing the ordered list created in step (j) as a keratinocyte instance on the at least one computer readable medium;
(l) constructing a data architecture of stored keratinocyte instances by repeating (g) through (k), wherein the at least one perturbagen of step (h) is different for each keratinocyte instance;
(m) querying the data architectures of stored fibroblast and keratinocyte instances with at least one hair biology-relevant gene expression signature, wherein querying comprises comparing the at least one hair biology-relevant gene expression signature to each stored fibroblast and keratinocyte instance, wherein the hair biology-relevant gene expression signature represents genes differentially expressed in association with the at least one hair biology condition;
(n) assigning a connectivity score to each of the instances; and
(o) preparing a hair/scalp care composition comprising at least one perturbagen, wherein the connectivity score of the instance associated with the at least one perturbagen has a negative correlation.
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Abstract
Provided are methods and systems for determining functional relationships between a cosmetic agent and a hair biology condition of interest. Also provided are methods and systems for identifying cosmetic agents that affect a hair biology condition, as well as the use of agents identified by such methods and systems for the preparation of cosmetic compositions, personal care products, or both.
118 Citations
43 Claims
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1. A method for constructing a data architecture for use in identifying connections between perturbagens and genes associated with one or more cosmetic hair biology conditions and preparing a hair/scalp care composition, comprising:
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(a) providing a gene expression profile for a control human fibroblast cell; (b) generating a gene expression profile for a human fibroblast cell exposed to at least one perturbagen by extracting a biological sample from the exposed cell and subjecting the biological sample to microarray analysis via a microarray scanner; (c) identifying genes differentially expressed in response to the at least one perturbagen by comparing the gene expression profiles of (a) and (b); (d) creating an ordered list comprising identifiers representing the differentially expressed genes, wherein the identifiers are ordered according to the differential expression of the genes; (e) storing the ordered list as a fibroblast instance on at least one computer readable medium; and (f) constructing a data architecture of stored fibroblast instances by repeating (a) through (e), wherein the at least one perturbagen of step (a) is different for each fibroblast instance; (g) providing a gene expression profile for a control human keratinocyte cell; (h) generating a gene expression profile for a human keratinocyte cell exposed to at least one perturbagen by extracting a biological sample from the exposed cell and subjecting the biological sample to microarray analysis via a microarray scanner; (i) identifying genes differentially expressed in response to the at least one perturbagen by comparing the gene expression profiles of (g) and (h); (j) creating an ordered list comprising identifiers representing the differentially expressed genes, wherein the identifiers are ordered according to the differential expression of the genes identified in (i); (k) storing the ordered list created in step (j) as a keratinocyte instance on the at least one computer readable medium; (l) constructing a data architecture of stored keratinocyte instances by repeating (g) through (k), wherein the at least one perturbagen of step (h) is different for each keratinocyte instance; (m) querying the data architectures of stored fibroblast and keratinocyte instances with at least one hair biology-relevant gene expression signature, wherein querying comprises comparing the at least one hair biology-relevant gene expression signature to each stored fibroblast and keratinocyte instance, wherein the hair biology-relevant gene expression signature represents genes differentially expressed in association with the at least one hair biology condition; (n) assigning a connectivity score to each of the instances; and (o) preparing a hair/scalp care composition comprising at least one perturbagen, wherein the connectivity score of the instance associated with the at least one perturbagen has a negative correlation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. A method for constructing a data architecture for use in identifying connections between cosmetic perturbagens and genes associated with cosmetically improving hair biology and preparing a hair/scalp care composition, comprising:
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(a) providing a gene expression profile for a control fibroblast human cell; (b) generating a gene expression profile for a human fibroblast cell exposed to at least one perturbagen by extracting a biological sample from the exposed cell and subjecting the biological sample to microarray analysis via a microarray scanner; (c) identifying genes differentially expressed in response to at least one perturbagen by comparing the gene expression profiles of (a) and (b); (d) creating an ordered list comprising identifiers representing the differentially expressed genes, wherein the identifiers are ordered according to the differential expression of the genes; (e) storing the ordered list as a fibroblast instance on at least one computer readable medium; (f) constructing a data architecture of stored instances by repeating (a) through (e), wherein the at least one perturbagen of step (b) is different qualitatively or quantitatively for each instance; (g) repeating steps (a) through (f) for a control human keratinocyte cell and human keratinocyte cells; (h) constructing a data architecture incorporating both the fibroblast and the keratinocyte instances; (i) querying the data architecture of stored instances with at least one hair biology relevant gene expression signature, wherein querying comprises comparing the hair biology-relevant gene expression signature to each stored cell instance, wherein the hair biology-relevant expression signature represents genes differentially expressed in a human tissue affected with a cosmetic hair biology condition or genes differentially expressed in cells treated with at least one benchmark agent having known efficacy in treating a cosmetic hair condition; (j) assigning a connectivity score to each of the instances; and (k) preparing a hair/scalp care composition comprising at least one perturbagen, wherein the connectivity score of the instance associated with the at least one perturbagen has a negative correlation. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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Specification