Hemangio-colony forming cells

  • US 9,938,500 B2
  • Filed: 09/29/2015
  • Issued: 04/10/2018
  • Est. Priority Date: 04/14/2006
  • Status: Active Grant
First Claim
Patent Images

1. A method, comprising:

  • (a) culturing human embryonic stem cells in serum free media in the presence of vascular endothelial growth factor (VEGF) and bone morphogenic protein 4 (BMP-4) in an amount sufficient to induce the differentiation of said embryonic stem cells into embryoid bodies; and

    (b) adding at least two first growth factors selected from the group consisting of;

    basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), bone morphogenic protein 4 (BMP-4), stem cell factor (SCF), thrombopoietin (TPO), Flt-3L (FL), HOX protein, and erythropoietin (EPO) to the culture of embryoid bodies(c) disaggregating the embryoid bodies into single cells; and

    (d) adding erythropoietin (EPO) to said single cell culture in an amount sufficient to expand CD34


    human hemangio-colony forming cells in the culture;

    wherein the embryonic stem cells, embryoid bodies, and CD34


    hemangio-colony forming cells are continuously grown in serum-free media to generate CD34


    human hemangio-colony forming cells wherein the erythropoietin (EPO) is added to the single cell culture in step (d) after 72 hours of initiating of the culturing of step (a), and optionally, added multiple times throughout step (d).

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