Soybean event 127 and methods related thereto

• US 9,961,848 B2
• Filed: 04/06/2015
• Issued: 05/08/2018
• Est. Priority Date: 01/07/2009
• Status: Expired due to Fees

• Current Assignee: BASF Agrochemical Products BV (BASF SE), Empresa Brasileira de Pesquisa Agropecuária
• Original Assignee: BASF Agrochemical Products BV (BASF SE), Empresa Brasileira De Pesquisa Agropecuã¡Ria - Embrapa.
• Inventors: Carlson, Dale, Aragao, Francisco Jose Lima, Arias, Carlos Alberto Arrabal, Louzano, Luiz, Luzzi, Bruce M., Malefyt, Tim, Filho, Elibio Leopoldo Rech, Tan, Siyuan, Ulbrich, Adolfo, Yotsumoto, Tadashi, Linemann, Ute
• Primary Examiner(s): Horlick, Kenneth R
• Application Number:
  US14/679,647
• Publication Number:
  US 20150250129A1
• Time in Patent Office: 1,128 Days
• Field of Search: 435 611, 435 612, 435 912
• CPC Class Codes: C12N 15/8271 for stress resistance, e.g. heavy metal resistance
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae

Abstract

Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location comprises genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO:5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.

Claims

1. A method for identifying an event 127 soybean plant, comprising:

(a) forming a mixture comprising a biological sample containing soybean DNA and a first and second nucleic acid primer capable of amplifying an event 127 specific nucleic acid molecule;

(b) reacting the mixture under conditions that allow the first and second nucleic acid primers to amplify an event 127 specific nucleic acid molecule; and

(c) detecting the presence of the amplified fragment nucleic acid molecule, wherein the presence of the soybean event 127 specific nucleic acid molecule indicates that the soybean plant is an event 127 soybean plant.

2. A method for identifying a soybean plant having an event 127 nucleic acid molecule, comprising:

(a) forming a mixture comprising a biological sample containing soybean DNA and a nucleic acid molecule probe that is capable of hybridizing to an event 127 specific nucleic acid molecule;

(b) reacting the mixture under conditions that allow the nucleic acid molecule probe to hybridize to an event 127 specific nucleic acid molecule; and

(c) detecting hybridization of the probe to the DNA, wherein the presence of hybridization of the nucleic acid molecule probe to the soybean DNA indicates the presence of an event 127 nucleic acid molecule.

3. A method for detecting the presence of an event 127 nucleic acid molecule in a biological sample, comprising:

(a) forming a mixture comprising a biological sample containing DNA and a first and second nucleic acid primer capable of amplifying an event 127 specific nucleic acid molecule;

(b) reacting the mixture under conditions that allow the first and second nucleic acid primers to amplify the nucleic acid molecule comprising an event 127 specific nucleic acid molecule; and

(c) detecting the presence of the amplified nucleic acid molecule, wherein the presence of the event 127 specific nucleic acid molecule indicates that the sample contains event 127 nucleic acid molecule.

4. The method of claim 3, wherein the biological sample is obtained from a soybean plant.

5. A method for detecting an event 127 nucleic acid molecule in a biological sample comprising:

(a) forming a mixture comprising a biological sample containing DNA and a nucleic acid probe capable of hybridizing to an event 127 specific nucleic acid molecule;

(b) reacting the mixture under conditions that allow the probe to hybridize to an event 127 specific nucleic acid molecule; and

(c) detecting the presence of a hybridized nucleic acid molecule, wherein the presence of the event 127 specific nucleic acid molecule indicates that the sample contains event 127 nucleic acid molecule.

6. The method of claim 5, wherein the biological sample is obtained from a soybean plant.