Method for manufacturing multiple-diagnosis membrane sensor by using screen printing

• US 9,970,933 B2
• Filed: 10/05/2012
• Issued: 05/15/2018
• Est. Priority Date: 10/06/2011
• Status: Active Grant

• Current Assignee: Ingibio Ltd.
• Original Assignee: Ingibio Ltd.
• Inventors: Kim, Min-Gon, Joung, Hyou-Arm, Ahn, Jun-Hyoung, Suk, Ho-Jun
• Primary Examiner(s): Long, Scott
• Assistant Examiner(s): HOLLINDEN, GARY E
• Application Number:
  US14/350,278
• Publication Number:
  US 20140287439A1
• Time in Patent Office: 2,048 Days
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: G01N 33/56911 Bacteria
• CPC Class Codes: G01N 33/56916 Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia

Abstract

Provided is a method for manufacturing a multiple-diagnosis membrane sensor provided with multiple channels by using screen printing, and more specifically, to a method for manufacturing a membrane sensor capable of performing multiple-diagnosis by screen-printing hydrophobic ink on a membrane to form multiple channels.

The membrane sensor according to the present invention may enable mass-production of sensors and secure reliability of detection by forming the plurality of channels on the membrane by a simple method.

Claims

1. A method for manufacturing a sensor using a screen-printing, the method comprising:

screen printing hydrophobic ink at a thickness of 5 to 10 μ

m onto a membrane in which a water soluble sample flows to form a pattern comprising multiple channels,

wherein

the hydrophobic ink comprises one or more of a silver paste, a carbon paste, a platinum paste, a ceramic paste, or a wax,

the hydrophobic ink comprises one or more organic solvents selected from alcohols having 1 to 6 carbon atoms, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), or acetone, and

the membrane comprises one or more of nitrocellulose, nylon, polysulfone, polyethersulfone, or polyvinylidene fluoride (PVDF).

2. A sensor, comprising:

a membrane; and

multiple channels formed by screen printing hydrophobic ink having a thickness of 5 to 10 μ

m on a surface of the membrane,

wherein

the membrane is configured to accommodate a lateral flow of a water soluble sample,

the hydrophobic ink comprises one or more of a silver paste, a carbon paste, a platinum paste, a ceramic paste, or a wax,

the hydrophobic ink comprises one or more organic solvents selected from alcohols having 1 to 6 carbon atoms, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), or acetone, and

the membrane comprises one or more of nitrocellulose, nylon, polysulfone, polyethersulfone, or polyvinylidene fluoride (PVDF).

3. The sensor of claim 2, further comprising a sample pad, a conjugation pad, and an absorption pad on the membrane.

4. The sensor of claim 3, wherein the sample pad or the absorption pad comprises one or more of cellulose, polyester, polypropylene, or a glass fiber.

5. The sensor of claim 3, wherein the conjugation pad comprises one or more of cellulose, polyester, polypropylene, glass fiber, nitrocellulose, nylon, polysulfone, polyethersulfone, or polyvinylidene fluoride (PVDF).

6. The sensor of claim 3, wherein the conjugation pad is treated with a signal generation material or a conjugate of a material selectively binding to an analyte and the signal generation material.

7. The sensor of claim 6, wherein the signal generation material comprises one or more of metal nanoparticles, quantum dot nanoparticles, magnetic nanoparticles, an enzyme, an enzyme substrate, an enzyme reactant, a light absorbent material, a fluorescent material, or a luminescent material.

8. A method of analyzing a biological sample, the method comprising:

injecting the biological sample into a sensor comprising a membrane and multiple channels formed by screen printing hydrophobic ink having a thickness of 5 to 10 μ

m on a surface of the membrane; and

measuring one or more analytes of the biological sample,

wherein

the hydrophobic ink comprises one or more of a silver paste, a carbon paste, a platinum paste, a ceramic paste, or a wax,

the hydrophobic ink comprises one or more organic solvents selected from alcohols having 1 to 6 carbon atoms, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), or acetone, and

the membrane comprises one or more of nitrocellulose, nylon, polysulfone, polyethersulfone, car polyvinylidene fluoride (PVDF).

9. The method of analyzing the biological sample of claim 8, further comprising measuring electrochemical signals between the multiple channels of the sensor.