Marker peptide for determining risk of hyperglycemia and use thereof

• US 9,970,946 B2
• Filed: 12/10/2013
• Issued: 05/15/2018
• Est. Priority Date: 12/20/2012
• Status: Active Grant

• Current Assignee: Lion Corporation
• Original Assignee: Lion Corporation
• Inventors: Uchiyama, Chiyoko, Kurita, Kei
• Primary Examiner(s): Russel, Jeffrey E.
• Application Number:
  US14/438,479
• Publication Number:
  US 20150285821A1
• Time in Patent Office: 1,617 Days
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: G01N 2333/47 Assays involving proteins of known structure or function as defined in the subgroups
• CPC Class Codes: G01N 2333/8139 Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
• CPC Class Codes: G01N 2800/042 Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
• CPC Class Codes: G01N 2800/50 Determining the risk of developing a disease
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere

Abstract

The object of the present invention is to provide a marker substance capable of determining a risk of hyperglycemia in vivo with high accuracy. The present invention provides the following: a marker peptide for determining the risk of hyperglycemia; an antibody or an aptamer bound to the marker peptide for determining the risk of hyperglycemia; a microarray wherein the antibody or the aptamer to be bound to the marker peptide for determining the risk of hyperglycemia has been immobilized onto a carrier; a method of determining the risk of hyperglycemia wherein an amount or the presence/absence of the marker peptide for determining the risk of hyperglycemia is measured in a biological sample collected from a subject; and a kit for determining the risk of hyperglycemia comprising the antibody or the aptamer, or the microarray.

Claims

1. A method of detecting a level of at least one peptide fragment selected from the group consisting of amino acids 25 to 39 of SEQ ID NO:

1, amino acids 59 to 83 of SEQ ID NO;

2, and amino acids 337-343 of SEQ ID NO;

3, the method comprising;

measuring the level of the at least one peptide fragment in a sample obtained from a subject.

2. The method according to claim 1, wherein the measuring comprises measuring by a mass spectrometry;

an immunoassay using an antibody or aptamer that binds to the at least one peptide fragment;

or an immunoassay using a microarray wherein an antibody or an aptamer that binds to the at least one peptide fragment is immobilized on a carrier.

3. The method according to claim 1, wherein the sample is saliva.

4. The method according to claim 1, wherein the at least one peptide fragment is amino acids 25 to 39 of SEQ ID NO:

1.

5. The method according to claim 1, wherein the at least one peptide fragment is amino acids 59 to 83 of SEQ ID NO:

2.

6. The method according to claim 1, wherein the at least one peptide fragment is amino acids 337-343 of SEQ ID NO:

3.

7. The method according to claim 1, wherein the subject is human.

8. The method according to claim 1, comprising measuring the level of at least two of the peptide fragments.

9. The method according to claim 1, wherein the subject has a risk of hyperglycemia.

10. The method according to claim 1, wherein the subject has no risk of hyperglycemia.