Arabinoxylan degrading enzymes

Arabinoxylan degrading enzymes

  • CN 100,415,889 C
  • Filed: 08/28/1995
  • Issued: 09/03/2008
  • Est. Priority Date: 08/26/1994
  • Status: Active Grant
First Claim
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1. contain coding and have (1,4)-β

  • -D-araboxylan arbinofuranose hydrolytic enzyme activities, but the dna fragmentation that does not have the nucleotide sequence of endo-xylanase and α

    -active polypeptide of arbinofuranose glycosides or its polypeptide precursor is characterized in that described nucleotide fragments is selected from;

    (a) coding has the polypeptide of the aminoacid sequence of amino acid/11 to 306 representative among the SEQ ID NO;

    5 or amino acid-27 nucleotide fragments to the polypeptide precursor of the described polypeptide of 306 representatives;

    (b) coding has the polypeptide of the aminoacid sequence of amino acid/11 to 306 representative among the SEQ ID NO;

    7 or amino acid-27 nucleotide fragments to the polypeptide precursor of the described polypeptide of 306 representatives;

    (c) nucleotide sequence of Nucleotide 823 to 1818 representatives among Nucleotide 784 to 1779 or the SEQ ID NO;

    7 among the SEQ ID NO;

    5;

    (d) coding has (1,4)-β

    -D-araboxylan arbinofuranose hydrolytic enzyme activities, but the nucleotide sequence that does not have endo-xylanase and α

    -active polypeptide of arbinofuranose glycosides, this nucleotide sequence can be under hybridization conditions with SEQ ID NO;

    5 in Nucleotide 784 to 1779 representatives dna fragmentation or with SEQ ID NO;

    7 in the dna fragmentation hybridization of Nucleotide 823 to 1818 representatives, described hybridization conditions is included among 5 * SSC/0.1%SDS and washed 30 minutes 65 ℃

    of first time, in 2 * SSC/0.1%SDS, washed 30 minutes subsequently 65 ℃

    of second time, next in 0.1 * SSC/0.1%SDS, washed for the third time 30 minutes in 65 ℃

    , then in 0.1 * SSC in 65 ℃

    of the 4th washings 30 minutes.

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