Cost effective media for large scale insect cell culture

Cost effective media for large scale insect cell culture

  • CN 100,475,955 C
  • Filed: 06/22/2001
  • Issued: 04/08/2009
  • Est. Priority Date: 06/23/2000
  • Status: Active Grant
First Claim
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1. a scale operation is used for the method for the liplid emulsions of cell culture medium, and this method comprises:

  • A) the haddock liver oil fatty acid methyl ester of Tween 80, the 1.0g/L of 2.5g/L, the cholesterol of 0.45g/L and the alpha-tocopherol of 0.2g/L are mixed,B) the described mixture to 50 of heating is ℃

    -60 ℃

    ,C) continue to stir described mixture until the solid dissolving and obtain liquid phase with magnetic stirring bar,D) continue to heat and mix described mixture, slowly add 0.5ml water simultaneously, then add the 1.3ml propyl alcohol,E) described mixture is cooled to room temperature,F) add the 1.7ml propyl alcohol while mixing, obtain liquid phase once more,G) dropwise add the 8.3ml water or the 1%Pluronic F-68 aqueous solution while mixing,H) increase stir speed (S.S.), add the 0.8ml water or the 1%Pluronic F-68 aqueous solution again,I) slowly heat described mixture while stirring 15 minutes, keep temperature to be lower than 40 ℃

    simultaneously,J) add the entry or the 1%Pluronic F-68 aqueous solution again to increase volume of mixture to 1.0L.

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