Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors

Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors

CN
  • CN 100,491,519 C
  • Filed: 01/26/2001
  • Issued: 05/27/2009
  • Est. Priority Date: 01/28/2000
  • Status: Active Grant
First Claim
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1. method that contains the lipid of polyene fatty acid from thraustochytriales microorganisms production, described microorganism can produce many unsaturated lipids of the TL at least 15% that accounts for its generation, and this method is included in the fermentation of carrying out described microorganism in the fermention medium that contains non-pure carbon source and limiting nutrient source;

  • Wherein said method comprises adds non-pure carbon source and limiting nutrient source in the fermention medium that comprises described microorganism, the speed of interpolation is enough to improve the biomass density of this fermention medium to the 100g/L at least based on dry cell weight;

    AndWherein said have based on dry cell weight at least the substratum fermentation of 100g/L biomass density produce the lipid that contains polyene fatty acid.

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