Detection method based on solution

Detection method based on solution

  • CN 100,570,366 C
  • Filed: 07/31/2003
  • Issued: 12/16/2009
  • Est. Priority Date: 08/01/2002
  • Status: Active Grant
First Claim
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1. test kit comprises:

  • (a) at least a reagent is used to detect at least a characteristic marker molecules of medical science correlation behavior, and wherein said marker molecules is selected from p13.5, p14, p15, p16 INK4a, p19, p21, p27, p53, pRb, p14ARF, cyclin A, cyclin B, cyclin E, MDM-2, MCM2, MCM5, MCM6, CDC2, CDC6, Id1, osteonectin, GRP, kidney dipeptidase, TGF β

    II acceptor, the label relevant, claudin-1, MN with HPV, her2/neu, bcl-2, EGF-acceptor, the label of assignor'"'"'s papilloma virus infection or Ki67;

    And (b) at least a reagent that is used to detect at least a standard marker molecules, this standard label has the characteristic of following at least a parameter;

    Whether have specific cell type or differentiation model among the cell that occurs in sample solution, and whether the cell that occurs in sample solution exist specific multiplication characteristic, wherein said standard marker molecules is selected from the keeping gene, PCNA, inhibin, CK20 cell surface antigen, glycoprotein, people'"'"'s epitheliosis 40kD albumen, people'"'"'s epithelium antigen 34+39KD, cytokeratin 18, cytokeratin 8, mucoitin antigen, the GaLNac transferase, few polysaccharide transferase, lectin, Plakophilin, vimentin, haemoglobin, CD16, CD56, CD68, CD19, CD20, CD3, CD4, CD8, CEA, NMP22, BTA, PreproGRP, PCNA, cytokeratin, E-cadherin, the involucrum involucre, the plasminogen activators of urokinase shape, SCCA, α

    -connection is plain, and β

    -connection is plain, and γ

    -connection is plain, CK8, CK13, CK18 or Ep-Cam;

    (c) be used to dissolve the damping fluid of sample.

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