SELECTIVE-BREEDING AND LEAVENING TECHNOLOGIES FOR PRODUCTION-INCREASING YIELD BACTERIA OF CROPS

SELECTIVE-BREEDING AND LEAVENING TECHNOLOGIES FOR PRODUCTION-INCREASING YIELD BACTERIA OF CROPS

  • CN 1,008,534 B
  • Filed: 09/28/1988
  • Issued: 06/27/1990
  • Est. Priority Date: 09/28/1988
  • Status: Active Grant
First Claim
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1. , a kind of solid fermentation method of crops output increasing bacteria, the used fermentation strain of this method is in plant materials or crops output increasing bacteria YIB (a kind of waxy Bacillus bacterial strain of obtaining of body surface separation screening, CGMCC0137, preservation date on September 20th, 1988), it is characterized in that:

  • A, earlier with careless carbon drying, then through mechanical disintegration be fineness 30 orders/cun, stir adding water, after water content reached 40%, pack was sent under 121 ℃

    of conditions again, the 30 fens kinds of sterilizing are made the sterile solid culture material,B, elder generation connect the solid medium enlarged culturing with former actication of culture, in that the solid culture bacterial classification is inserted liquid medium, carry out shaking table and cultivate into original bacteria liquid, add the sterilized water dilution then, make bacterium liquid,C, the bacterium liquid made is inserted in the sterile solid culture material of pH7-7.4, every gram solid culture medium inoculum size is 4 * 10 4More than the cfv/g, after the solid culture medium inoculation, send into culturing room and cultivate into the solid yield increasing fungus,, be product again through mark of parcels, D, above-mentioned substratum, nutrient solution, culture material are all taked high pressure steam sterilization, promptly at 121 ℃

    , pressure 0.5kgF/cm 2Sterilization is 30 minutes under the condition.Strain expanded culture, the temperature that shaking table is cultivated and culturing room cultivates are 22-35 ℃

    , cultivated 30-40 hour, E, solid culture material formula are;

    careless carbon 90g, water 10g, pH value 7.

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