Preparing method of heterotransplantation of bones

Preparing method of heterotransplantation of bones

  • CN 101,032,631 A
  • Filed: 04/06/2007
  • Published: 09/12/2007
  • Est. Priority Date: 04/06/2007
  • Status: Active Application
First Claim
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1. , a kind of preparation method of heterotransplantatioof of bones is a raw material with the animal bone, it is characterized in that may further comprise the steps:

  • (1) removes bone marrow and hemocyteThe animal bone of obtaining under the aseptic condition is used high-voltage pulse distilled water or normal saline flushing under normal pressure, room temperature and aseptic condition, washing time exceeds with bone marrow and the hemocyte of removing in bone and the pulp cavity thereof;

    (2) ether defatting and flushingAt normal temperatures and pressures step (1) is removed the ether defat that the animal bone of bone marrow and hemocyte places mass concentration 95%~

    100%, the consumption of ether exceeds to flood processed animal bone, the time of ungrease treatment exceeds with the fat of removing in the animal bone, after the ungrease treatment, remove ether with aseptic water washing;

    (3) super deep-frozenWill be through animal bone liquid nitrogen freezing under normal pressure of step (2) processing, cooling time was at least 1 month, to destroy osteocyte, reduced antigenicity;

    (4) radiation gammaWith the animal bone behind the super deep-frozen of step (3) under normal pressure, room temperature with radiation gamma sterilization with further destroy osteocyte, promptly obtain to have removed the heterotransplantatioof of bones of heteroantigen.

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