Recombination adenovirus for curing overexpression proto-oncogene neu/erbB2 malignancy

Recombination adenovirus for curing overexpression proto-oncogene neu/erbB2 malignancy

  • CN 101,054,596 A
  • Filed: 04/03/2007
  • Published: 10/17/2007
  • Est. Priority Date: 04/03/2007
  • Status: Active Application
First Claim
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1. , the Humanized monoclonal antibodies strand variable region of a kind of coexpression proto-oncogene neu/erbB2 and the recombinant adenovirus of Mda-7.The adenovirus carrier that the present invention uses is the product A dEasy-1 carrier of Stratagene company, is the adenovirus carrier of E1 and E3 district disappearance.The expression cassette that the present invention makes up is made up of Humanized monoclonal antibodies strand variable region, internal ribosome binding site (IRES) fragment, Mda-7 and the SV40 poly VITAMIN B4 of CMV mover, proto-oncogene neu/erbB2, and its principal character is:

  • A). the Humanized monoclonal antibodies of proto-oncogene neu/erbB2 only is made of antibody variable region;

    B). in the Humanized monoclonal antibodies variable region sequences of proto-oncogene neu/erbB2, variable region of light chain and variable region of heavy chain link by amino acid polypeptide-G-G-G-G-G-S-(GS1), at its N-end with restriction endonuclease Nhe1 and C-end with restriction endonuclease Not1 fragment assembly in pIRES plasmid upstream;

    C). the 72nd amino acids of people Mda-7 is mutated into Val by Ala, and other amino acid are formed and put in order constant;

    D) the .Mda-7 gene at its N-end with restriction endonuclease Smal and C-end with restriction endonuclease Xba 1 fragment assembly in pIRES plasmid downstream;

    After reorganization, this expression cassette is positioned at the E1 disappearance district of adenovirus carrier.This recombinant adenovirus has the Humanized monoclonal antibodies strand variable region of coexpression neu/erbB2 and the effect of Mda-7, thereby has stronger apoptosis function, the wider biological function of anticancer spectrum.

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