Cultivation of primate embryonic stem cells

Cultivation of primate embryonic stem cells

  • CN 101,072,868 B
  • Filed: 09/27/2005
  • Issued: 04/15/2015
  • Est. Priority Date: 09/28/2004
  • Status: Active Grant
First Claim
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1. a cell culture medium, substantially not containing mammalian fetal serum, containing amino acid, VITAMIN, mineral substance, transferrin, Regular Insulin, the fibroblast growth factor of albumin and abbreviation FGF, described FGF is selected from FGF2, FGF4, and FGF17, its concentration is at least the minimal maintenance concentration that can activate fibroblast growth factor signal transduction acceptor, described minimal maintenance concentration is 100ng/ml, described substratum can not contain serum, support when not containing feeder cell and without the need to making substratum contact feeder cell that the euploid embryonic stem cell that do not break up of H1 human embryonic stem cell is cultivated and at least 6 generations of propagation.

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