Method for preparing cadmium selenide quantum dots by using microbial cells

Method for preparing cadmium selenide quantum dots by using microbial cells

  • CN 101,096,687 B
  • Filed: 07/11/2007
  • Issued: 08/29/2012
  • Est. Priority Date: 07/11/2007
  • Status: Active Grant
First Claim
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1. method of utilizing yeast cell to prepare CdSe quantum dots, this method comprises the following steps:

  • The cultivation of A, yeast cell;

    inoculate in single bacterium colony to the yeast cell substratum, substratum is the YPD substratum, and is when culturing cell grows to the stable growth phase, for use;

    B, the selenizing of yeast cell is handled;

    in the yeast cell suspension that steps A prepares, add Sodium Selenite, its use final concentration be 0.1 mmole/liter~

    10 mmoles/liter, continue then to cultivate 1~

    36 hour;

    C, the cell after the selenizing is carried out the cadmium processing and continue to cultivate;

    the cell after 8000 rev/mins of centrifugal collections are handled through step B Sodium Selenites;

    And be suspended in again in the fresh yeast culture base;

    And in substratum, add Cadmium chloride fine powder;

    Its use final concentration be 0.1 mmole/liter~

    10 mmoles/liter, continue to cultivate 1~

    24 hour, quantum dot is synthetic in cell immediately;

    The extraction of CdSe quantum dot in D, the cell;

    make lysis, discharge cell content;

    The purifying of E, CdSe quantum dot;

    extracellular quantum dot passes through gradient centrifugation;

    1000 rev/mins, 4000 rev/mins, 16000 rev/mins, 25000 rev/mins, 30000 rev/mins;

    Molecular sieve and high speed centrifugation carry out purifying for 45000 rev/mins, obtain the CdSe quantum dot.

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