Fast-culturing susceptibility and micro-detection method for mycobacteria and device thereof

Fast-culturing susceptibility and micro-detection method for mycobacteria and device thereof

  • CN 101,130,808 A
  • Filed: 07/27/2007
  • Published: 02/27/2008
  • Est. Priority Date: 07/27/2007
  • Status: Active Application
First Claim
Patent Images

1. the trace detection method of mycobacterium fast-culturing susceptibility and evaluation,after the sample disposal, be inoculated in substratum, through cultivating, the antiacid mycobacterium that will grow in the Biosafety cupboard is taken out, and makes the bacteria suspension of normality;

  • 2. in the Biosafety cupboard, the special-purpose microtest plate loam cake that is provided with is opened, each hole has added substratum and relative medicine in advance in the plate, other has positive control hole (contain substratum and do not have medicine) and negative control hole (no substratum does not have medicine), the bacteria suspension that in each hole, adds the 0.1ml normality, loam cake is built, and will be sealed between lid and bottom;

    3. each plate is put into the wet box of sealing, placed in 37 ±

    2 ℃

    of constant incubators and cultivate;

    It is characterized in that 4. every day or take out culture plate every other day, be placed on the live bacteria direct-view amplification system and observe;

    From the viewing window of each bottom, hole of special-purpose culture plate, by optical amplification system, gather the bacterial growth image, and compare with negative and positive control wells image, the similar resistance that is judged to the positive control hole, on the contrary be responsive.

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