Method for producing polyunsaturated C20 and C22fatty acids with at least four double bonds in transgenic plants

Method for producing polyunsaturated C20 and C22fatty acids with at least four double bonds in transgenic plants

  • CN 101,146,911 A
  • Filed: 03/21/2006
  • Published: 03/19/2008
  • Est. Priority Date: 03/22/2005
  • Status: Active Application
First Claim
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1. be used in transgenic plant, producing how unsaturated C with at least 4 two keys 20-or C 22The method of-lipid acid, the content of described lipid acid account at least 15% of triglyceride level total content in the transgenic plant by weight, and wherein said method is characterised in that and comprises following step:

  • A) will comprise the nucleic acid construct that coded delta 6-desaturase, Δ

    6-prolong the nucleotide sequence of enzyme and Δ

    5-desaturase and import transgenic plant, orB) will comprise the nucleic acid construct that coded delta 6-desaturase, Δ

    6-prolong the nucleotide sequence of enzyme, Δ

    5-desaturase, Δ

    12-desaturase and ω

    3-desaturase and import transgenic plant, orC) will comprise coded delta 6-desaturase, Δ

    6-and prolong the nucleic acid construct that enzyme, Δ

    5-desaturase, Δ

    5-prolong the nucleotide sequence of enzyme and Δ

    4-desaturase and import transgenic plant, orD) will comprise coded delta 6-desaturase, Δ

    6-prolong nucleic acid construct that enzyme, Δ

    5-desaturase, Δ

    5-prolong the nucleotide sequence of enzyme, Δ

    4-desaturase, Δ

    12-desaturase and ω

    3-desaturase import transgenic plant andE) obtain oil and fat from this plant.

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