The compositions selected for transfectional cell metabolic and method

The compositions selected for transfectional cell metabolic and method

  • CN 101,193,918 B
  • Filed: 05/18/2006
  • Issued: 11/23/2016
  • Est. Priority Date: 05/18/2005
  • Status: Active Grant
First Claim
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1. , for producing a mammalian expression vector for heterologous polypeptide, it comprises:

  • Encode the polynucleotide of the 3-ketosteroid reductase as metabolic selection markers,Encode the polynucleotide of described heterologous polypeptide,For driving SV40 promoter and the origin of replication of the expression of the 3-ketosteroid reductase as metabolic selected marker, andCytomegalovirus main early promoter and enhancer at once for driving the expression of described heterologous polypeptide.

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