A method for preparing a stable immunogenic product comprising antigenic heterocomplexes of TNF alpha and a carrier protein

A method for preparing a stable immunogenic product comprising antigenic heterocomplexes of TNF alpha and a carrier protein

  • CN 101,222,941 A
  • Filed: 05/24/2006
  • Published: 07/16/2008
  • Est. Priority Date: 05/24/2005
  • Status: Active Application
First Claim
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1. be used to prepare the method for anti-TNF alpha immunogenic product, comprise step:

  • A) provide the solution that contains TNF α

    ;

    B) in the solution that contains TNF α

    of step a), add EDTA;

    C) in the solution that when step b) finishes, obtains, add carrier protein;

    D) in the liquid mixture that when step c) finishes, obtains, add glutaraldehyde;

    E) from the solution that when step d) finishes, obtains, remove the free molecule of glutaraldehyde and TNF α and

    described carrier protein;

    F) in the solution that when step e) finishes, obtains, add formaldehyde, and kept the existence of formaldehyde at 96 hours to 192 hours in the time range;

    G) in the solution that when step f) finishes, obtains, add the reagent of blocking with formaldehyde reaction;

    AndH) from step h) remove formaldehyde and blocking-up reagent the solution that obtains when finishing, thus obtain to contain the solution of above-mentioned anti-TNF alpha immunogenic product.

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