Chromosomal conformation chip capture (4C) measures

Chromosomal conformation chip capture (4C) measures

  • CN 101,238,225 B
  • Filed: 07/03/2006
  • Issued: 11/30/2016
  • Est. Priority Date: 07/04/2005
  • Status: Active Grant
First Claim
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1. the method by analyzing the frequency that target nucleotide sequences interacts with one or more purpose nucleotide sequences can obtainThe nucleotide sequence of the cyclisation arrived, said method comprising the steps of:

  • A () provides the sample of crosslinking DNA;

    B DNA that () cross-links with the first digestion with restriction enzyme;

    C () connects the nucleotide sequence of crosslinking;

    D () releases crosslinking;

    E () is with the second digestion with restriction enzyme nucleotide sequence;

    F () is by known for nucleotide composition one or more DNA sequence and at one or more purpose nucleotide sequence flanksAvailable one or more second digestion with restriction enzyme sites connect;

    Wherein the coupled reaction in step (f) causes the formation of DNA circle,The nucleotide sequence of wherein said cyclisation comprises the first and second nucleotide sequences, wherein the first and second nucleotide sequencesEach end separated by different restriction enzyme enzyme recognition site, and wherein said first nucleotide sequence is target nucleus glycosidesAcid sequence and described second nucleotide sequence are obtained by crosslinking genomic DNA.

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