Method for making mature insulin polypeptides

Method for making mature insulin polypeptides

  • CN 101,243,190 B
  • Filed: 08/15/2006
  • Issued: 05/13/2015
  • Est. Priority Date: 08/16/2005
  • Status: Active Grant
First Claim
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1. the method for acquaintance'"'"'s insulin analog is prepared into by cultivating the fungal cell that there is the DNA vector of the human insulin analogue precursor of following formula containing encoding,B(1-30)-X-X-Z-Y-Y-A(1-21)Wherein B (1-30) is the analogue of B-chain insulin human, A (1-21) is the analogue of A-chain insulin human, X-X and Y-Y is Lys-Arg, and Z is AspGlyLeuGly, wherein the analogue of insulin human A-and/or B-chain comprises 3 sudden changes of the sudden change Glu being included in A14 position, described Lys-Arg is cut in fungal cell, thus allows cell in substratum, secrete maturation, two chain human insulin'"'"'s analogue of correct processing.

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