Methods for the amplification, quantitation and identification of nucleic acids

Methods for the amplification, quantitation and identification of nucleic acids

  • CN 101,278,061 A
  • Filed: 08/31/2006
  • Published: 10/01/2008
  • Est. Priority Date: 09/01/2005
  • Status: Active Application
First Claim
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1. the method for the multiple selected nucleic acid molecule of amplification from the nucleic acid molecule storehouse, it comprises:

  • (a) the multiple selected nucleic acid molecule of amplification in first round multiplex amplification reaction, it is right that described reaction comprises multiple outside primer, every pair of primer has specificity to a kind of selected nucleotide sequence, wherein allows described amplified reaction to proceed between the amplicon significantly competition point is before taken place reactive component;

    With(b) take turns the described selected nucleic acid molecule that further increases in the amplified reaction a plurality of second, each reaction respectively comprises a part of finishing multiple reaction and at least one pair of the inner primer as template, every pair of primer has specificity to a kind of described selected nucleotide sequence, makes each second take turns respectively further subgroup in the described multiple selected nucleic acid molecule of amplification of reaction.

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