A kind of high vigor height product specificities sucrose isomerase produces the high-throughput screening method of bacterial strain

A kind of high vigor height product specificities sucrose isomerase produces the high-throughput screening method of bacterial strain

  • CN 101,289,687 B
  • Filed: 06/10/2008
  • Issued: 12/16/2015
  • Est. Priority Date: 06/10/2008
  • Status: Active Grant
First Claim
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1. high vigor height product specificities sucrose isomerase produces a high-throughput screening method for bacterial strain, it is characterized in that the method comprises the following steps:

  • (1) sucrose isomerase enzyme-producing bacteria to be screened access is contained in the microwell plate I of substratum, shaking culture;

    (2) by microwell plate I centrifugal segregation supernatant liquor, add sucrose solution and carry out enzymatic conversion reaction;

    (3) after conversion terminates, microwell plate I is centrifugal, is transferred in microwell plate II successively, adds colouring reagents, and detect light absorption value, filter out sucrose isomerase superior strain in microwell plate II after being diluted by the supernatant liquor in hole every in microwell plate I;

    (4) by sucrose isomerase superior strain amplification culture, add sucrose solution and carry out enzymatic conversion reaction, detect the proportion of products in sucrose inversion liquid with thin-layer chromatography, obtain the bacterial strain of high yield Palatinose or the bacterial strain of high yield trehalulose;

    The colouring reagents used during described thin-layer chromatography detects is aniline-pentanoic-phosphoric acid.

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