Process for extracting chlorogenic acid from Eupatorium adenophorum

Process for extracting chlorogenic acid from Eupatorium adenophorum

  • CN 101,302,156 A
  • Filed: 05/08/2007
  • Published: 11/12/2008
  • Est. Priority Date: 05/08/2007
  • Status: Active Application
First Claim
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1. , the extraction process of chlorogenic acid in a kind of Hemp Eupatorium comprises the steps:

  • 1) leaf with Hemp Eupatorium is ground into powder;

    2) with the Hemp Eupatorium leaf powder that obtains in the step

         1), adding volume ratio is the aqueous ethanolic solution of 20-60%, and with the hydrochloric acid adjust pH to 2-6;

    At 50-80 ℃

    , will mix liquid and decoct 2-5 hour;

    The Hemp Eupatorium leaf powder that is added and the weight ratio of aqueous ethanolic solution are 1;

    8-13;

    3) decompress filter leaches filtrate 1;

    Filter residue is added the aqueous ethanolic solution that volume ratio is 20-60% once more, and with the hydrochloric acid adjust pH to 2-6;

    At 50-80 ℃

    , will mix liquid and decoct 2-5 hour;

    4) decompress filter leaches filtrate 2;

    Filter residue is added the aqueous ethanolic solution that volume ratio is 20-60% once more, and with the hydrochloric acid adjust pH to 2-6;

    At 50-80 ℃

    , will mix liquid and decoct 2-5 hour;

    5) decompress filter leaches filtrate 3;

    Merge three times filtrate, evaporation removes and desolvates, and gained medicinal extract is dried up with pneumatic pump, obtains the thick product of chlorogenic acid;

    6) with the thick product of Amberlyst process purifying chlorogenic acid, the thick product of chlorogenic acid is dissolved in the ethanol, last sample in macroporous resin, with deionized water as elutriant, twice on mistake post;

    Except that desolvating, drying obtains the chlorogenic acid extract with the water elution thing.

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