Polynucleotide amplification

Polynucleotide amplification

  • CN 101,316,937 B
  • Filed: 11/29/2006
  • Issued: 05/25/2016
  • Est. Priority Date: 11/29/2005
  • Status: Active Grant
First Claim
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1. the method for a group polynucleotide molecule in amplification sample, is characterized in that comprising the following steps:

  • A) obtain RNA sample reverse transcription whole piece RNA molecule, thereby found full-length cDNAOr the sample of acquisition full-length cDNA, wherein said RNA sample is mRNA sample,B) after 3 '"'"' end, hold tailing by 3 '"'"' of the cDNA transcribing with polynucleotides tail,C) use pair for amplification primer amplification cDNA, wherein the 1 '"'"' primer and cDNA 5 '"'"'End hybridization, the 25 '"'"' upstream portion of primer pair polynucleotides tail and the 3 '"'"' polynucleotides of cDNA1 to 10 the nearest nucleotides of tail upstream is special.

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