Non-fluorescent energy transfer

Non-fluorescent energy transfer

  • CN 101,321,878 B
  • Filed: 10/05/2006
  • Issued: 03/27/2013
  • Est. Priority Date: 10/05/2005
  • Status: Active Grant
First Claim
Patent Images

1. one kind comprises at least one oligonucleotide or at least one polynucleotide and at least one biomolecules of non-fluorescence donor part basically, wherein said non-fluorescence donor basically partly comprises 4 '"'"', 5 '"'"'-dimethoxy-6-Fluoresceincarboxylic acid, 4 '"'"', 5 '"'"'-dimethoxy-CF, one or more in 6-carboxyl-amino pentachloro-fluorescein or 5-carboxyl-amino pentachloro-fluorescein, this basically non-fluorescence donor part non-fluorescent energy can be transferred to this acceptor part when at least one acceptor part enough approaches with it, make non-fluorescent energy that this acceptor partial response accepts and emission of light, wherein this basically the peak value visible absorption of non-fluorescence donor part and this acceptor part differ 100nm or larger.

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