Conditionally immortalized long-term stem cells and methods of making and using such cells

Conditionally immortalized long-term stem cells and methods of making and using such cells

  • CN 101,330,830 A
  • Filed: 10/18/2006
  • Published: 12/24/2008
  • Est. Priority Date: 10/18/2005
  • Status: Active Application
First Claim
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1. the method for a working condition immortalization adult stem cell comprises:

  • A) the adult stem cell colony of acquisition amplification;

    B) promote cell survival and the proto-oncogene of propagation or the described stem cell of nucleic acid molecules transfection of its bioactive fragment or homologue with comprising, wherein said proto-oncogene is derivable;

    C) the described stem cell of nucleic acid molecules transfection of the apoptotic protein that suppresses this cell with encoding;

    WithD) in the presence of the stem cell factor combination and make proto-oncogene keep the transfected cell of amplification under the active condition, with working condition immortalization adult stem cell.

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