Control for nucleic acid testing

Control for nucleic acid testing

  • CN 101,331,237 A
  • Filed: 12/19/2006
  • Published: 12/24/2008
  • Est. Priority Date: 12/21/2005
  • Status: Active Application
First Claim
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1. one kind is used for detecting the method whether sample that contains target biological molecules in suspection exists target biological molecules, said method comprising the steps of:

  • A) wherein this step by step a1) or a2) formA1) the internal contrast biomolecules is added to-suspect the sample contain target biological molecules and-do not contain the negative control sample of target biological molecules,-contain target biological molecules positive control sample and-contain the reagent control sample of target biological moleculesA2) the internal contrast biomolecules is added to-suspect the sample contain target biological molecules and-do not contain target biological molecules the negative control sample and-contain the positive control sample of target biological molecules,And the reagent that comprises target biological molecules control sample is provided,B) randomly by the described biomolecules of sample purifying of step a), obtaining to comprise the sample of purified biomolecules,C) be determined at step a) or b) in each sample of obtaining internal contrast biomolecules signal and target biological molecules signal existence whether,D) following checking contains in suspection and has or do not exist the target biological molecules signal in the sample of target biological molecules;

    -be independent of internal contrast biomolecules signal the sample that has the situation inspection to suspect to contain target biological molecules the target biological molecules signal have a situation, or under the situation that does not have the target biological molecules signal, check the situation that exists of internal contrast biomolecules signal-check whether the negative control sample exists internal contrast biomolecules signal and whether do not have the target biological molecules signal,-check positive control sample whether have the target biological molecules signal and whether have internal contrast biomolecules signal andThere is situation in the target biological molecules signal of the reagent control sample in the step d) of the described method of-inspection, or checks whether the reagent control sample exists the target biological molecules signal and randomly check whether there is internal contrast biomolecules signal,E) whether the existence that detects target biological molecules is measured in step c) and the existence of the target biological molecules signal verified in step d) and internal contrast biomolecules signal or do not exist in the indication sample and have or do not exist target biological molecules thus.

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