Method for extracting polysaccharide and other multiple active components in boiling juice of trepang

Method for extracting polysaccharide and other multiple active components in boiling juice of trepang

  • CN 101,375,863 B
  • Filed: 09/17/2008
  • Issued: 07/31/2013
  • Est. Priority Date: 09/17/2008
  • Status: Active Grant
First Claim
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1. method of extracting polysaccharide and other multiple active components in the boiled trepang juice, it comprises following content:

  • A, with boiled trepang juice with 100 purpose strainer filterings, the filtrate of collecting is passed into carries out centrifugalize in the centrifuge, collect filtrate;

    The liquor filtrate of centrifugal filtration is fed the nanofiltration concentrating and desalinating that circulates in the ultra micro membrane separation plant, the molecule of molecular cut off more than 200, desalination to trapped fluid salinity is below 0.5%;

    Again the ultrafiltration trapped fluid is warming up to the boiling and kept 30 minutes, be cooled to 40~

    50 °

    of C then, transferring pH is 7.0~

    8.0, add hydrolysising protease, addition is 5~

    20g hydrolysising protease/1kg liquor, enzymolysis 2~

    3 hours, and it is above and keep carrying out in 15 minutes enzyme denaturing to be warming up to 80 °

    of C then, carry out centrifugalize again and filter, collect filtrate;

    B, the filtrate of collecting is passed into carries out the triple effect following current in the triple effect concentrator and concentrate, being concentrated into proportion is 1.15-1.25, obtains the thickness paste;

    C, the slowly edible ethanol of adding 95% and continuous stirring in concentrating the thickness paste that obtains, abundant mix homogeneously, finally make concentration of alcohol reach 60%-85%, left standstill then 12 hours, more stratified feed liquid is carried out centrifugalize and obtain precipitation, measure sedimentary salt content, carry out the precipitate with ethanol desalination as the edible ethanol that adds 60%-85% greater than 1%, centrifugalize obtains precipitation again, measures the precipitation salt content, be lower than 1% until salt content, otherwise continue precipitate with ethanol;

    D, the precipitation behind the precipitate with ethanol is carried out drying and pulverize, make pulverous boiled trepang juice active component.

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