A method for obtaining a xeno-free hBS cell line

A method for obtaining a xeno-free hBS cell line

  • CN 101,384,703 A
  • Filed: 10/06/2006
  • Published: 03/11/2009
  • Est. Priority Date: 10/07/2005
  • Status: Active Application
First Claim
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1. obtain the method for the hBS clone of no allos material, described method comprises step:

  • I) remove the inner cell mass that zona pellucida surrounds with the acquisition trophectoderm by the operation of no allos material from blastocyst,Ii) remove trophectoderm at least in part obtaining isolating inner cell mass cell by the operation of no allos material,Iii) the inner cell mass cell is placed on the people'"'"'s feeder layer in the substratum of no allos material,Iv) inner cell mass cell and people'"'"'s feeder cell are cultivated about 5 days altogether to about 50 days time bar in the substratum of no allos material,V) if any, the operation by no allos material discharges the inner cell mass cell or derives from its cell from the trophectoderm of hypertrophy,Vi) optionally, with inner cell mass cell or the cell transfer that derives from it to the Freshman feeder layer in the substratum of no allos material, thereby the hBS cell of the no allos material of acquisition,Vii) by in the substratum of no allos material, cultivating the hBS cell of breeding no allos material altogether, thereby obtain the hBS clone of no allos material with people'"'"'s feeder cell.

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