Isatis smalt particle content measuring method

Isatis smalt particle content measuring method

  • CN 101,403,731 B
  • Filed: 10/30/2008
  • Issued: 07/23/2014
  • Est. Priority Date: 10/30/2008
  • Status: Active Grant
First Claim
Patent Images

1. the method for isatis smalt particle content measuring, is characterized in that it is adenosine that content is controlled index, adopts high performance liquid chromatography to carry out assay;

  • High performance liquid chromatography comprises that chromatographic condition and system suitability, the preparation of reference substance solution are, the preparation of need testing solution and content limit;

    Described chromatographic condition and system suitability are;

    take octadecyl silane as filling agent;

    Take methanol-water as mobile phase, and the volume ratio of methyl alcohol and water is 14;

    86,12;

    88 or 16;

    84;

    Detect wavelength 260nm ±

    2nm, number of theoretical plate calculates and should be not less than 3000 by adenosine peak;

    The preparation method of described reference substance solution is;

    get adenosine reference substance appropriate, the methyl alcohol of take is made a certain proportion of solution as solvent;

    The preparation method of described need testing solution is;

    take 30% methyl alcohol as solvent, ultrasonic method preparation.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×