Isatis smalt particle content measuring method

Isatis smalt particle content measuring method

  • CN 101,403,731 B
  • Filed: 10/30/2008
  • Issued: 07/23/2014
  • Est. Priority Date: 10/30/2008
  • Status: Active Grant
First Claim
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1. the method for isatis smalt particle content measuring, is characterized in that it is adenosine that content is controlled index, adopts high performance liquid chromatography to carry out assay;

  • High performance liquid chromatography comprises that chromatographic condition and system suitability, the preparation of reference substance solution are, the preparation of need testing solution and content limit;

    Described chromatographic condition and system suitability are;

    take octadecyl silane as filling agent;

    Take methanol-water as mobile phase, and the volume ratio of methyl alcohol and water is 14;


    88 or 16;


    Detect wavelength 260nm ±

    2nm, number of theoretical plate calculates and should be not less than 3000 by adenosine peak;

    The preparation method of described reference substance solution is;

    get adenosine reference substance appropriate, the methyl alcohol of take is made a certain proportion of solution as solvent;

    The preparation method of described need testing solution is;

    take 30% methyl alcohol as solvent, ultrasonic method preparation.

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