Method of detecting variation and kit to be used therein

Method of detecting variation and kit to be used therein

  • CN 101,421,420 A
  • Filed: 07/27/2007
  • Published: 04/29/2009
  • Est. Priority Date: 08/08/2006
  • Status: Active Application
First Claim
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1. One kind the sudden change detection method, it is the method for the sudden change of the DNA in the test samples, it is characterized in that,Described sample is the sample that contains the non-detected object DNA that detected object DNA that detection site undergone mutation and described detection site do not undergo mutation,Described method comprises following (A)~

  • (E) operation,(A) in the sample that contains described DNA, add comprise with the detection of detected object sequence complementary polynucleotide with probe and with the operation of non-detected object sequence complementary polynucleotide, in the described operation;

    Described detected object sequence is described detected object DNA or its partial sequence, and it contains the described detection site of having undergone mutation,Described non-detected object sequence is described non-detected object DNA or its partial sequence, and it contains the described detection site of not undergoing mutation;

    (B) make the operation of described detection with probe and described DNA hybridization;

    (C), measure the operation of the signal change of following temperature variation for the hybridization organizer of described DNA and described detection usefulness probe;

    (D) analyze the operation that described signal changes, determines the Tm value;

    (E) determine that by described Tm value described detected object site has or not the operation of sudden change.

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