Method and system for screen selecting combination of ligand with receptor in active somatic cell

Method and system for screen selecting combination of ligand with receptor in active somatic cell

  • CN 101,432,624 A
  • Filed: 04/29/2007
  • Published: 05/13/2009
  • Est. Priority Date: 04/29/2006
  • Status: Active Application
First Claim
Patent Images

1. ClaimA kind of methods for detecting the part combined with cell-membrane receptor of I, it is characterised in that comprise the following steps:

  • Cell is incubated with dyestuff and part;

    WithThe interior endocytic vesicle containing dyestuff is detected in cell.2- the method as described in claim 1, it is characterised in that including after incubation, detection before rinsing cell, so as to remove extracellular dyestuff.3. the method as described in any claim in claim 1 and 2, wherein described dyestuff is fluorescent dye.4. the method as described in any claim in claims 1 to 3, described cell-membrane receptor is one kind in G G-protein linked receptors or tyrosine kinase receptor family.5. the method as described in any claim in Claims 1-4, it is characterised in that detected using microscope.6. method as claimed in claim 5, it is characterised in that described microscope is Laser Scanning Confocal Microscope.7. method as claimed in claim 6, it is characterised in that detected in one or more optics picture.8. the method as described in any claim in claim 3 to 7, it is characterised in that the step of this method further comprises obtaining the image of one or more intracellular interior endocytic vesicles containing dyestuff.9. the method as described in any claim in claim 3 to 8, it is characterised in that this method further comprises carrying out quantitative step containing endocytic vesicle in dyestuff to intracellular.10. the method as described in any claim in claim 1 to 9, it is characterised in that the further detecting step of this method is carried out by variable factor of the time.Methods of the II as described in any claim in claim 1 to 10, it is characterised in that operate this method in the single hole of a porous plate.12. method as claimed in claim 11, it is characterised in that described each step procedure is operated by automatics.13. method as claimed in claim 2, it is characterised in that this method still further comprises the following steps;

    Rinse after cell, with second of dyestuff and second of part incubated cell;

    With'"'"' in before detection, cell is rinsed to remove extracellular second of dyestuff and second of part.14. a kind of system for screening the part combined with cell membrane, it is characterised in that including:

    Automated fluid operation device;

    X-y directions objective table;

    Observe the microscope configuration of endocytic vesicle imaging in individual cells;

    WithControl the control device of X- y article carrying platforms and microscope movement.15. system as claimed in claim 14, the control device described in it also includes the software for being used to analyze interior endocytic vesicle.16. the system as described in any claim in claim 14 and 15, it is characterised in that described17. the system as described in any claim in claim 14 to 16, it is characterised in that described culture dish is porous plate.

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