Method for preparing citronellyl by bioconversion in non-aqueous phase system

Method for preparing citronellyl by bioconversion in non-aqueous phase system

  • CN 101,457,240 A
  • Filed: 12/30/2008
  • Published: 06/17/2009
  • Est. Priority Date: 12/30/2008
  • Status: Active Grant
First Claim
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1. , bio-transformation prepares the method for citronellyl acrylate in a kind of non-aqueous system, it is characterized in that the substrate lipid acid that participates in reacting adopts the solvent-free reaction system when adopting organic phase reaction system, the substrate lipid acid that participates in reaction to be the saturated medium chain fatty acid of carbon chain lengths C5~

  • C10 when being the high boiling longer chain fatty acid of the saturated short chain fatty acid of carbon chain lengths C2~

    C4 and C12;

    The biological catalyst that is adopted is zhizopchin (Rhizopuschinensis) CCTCC M 201021 lipase;

    Technology is;

    Adopt the organic phase reaction system when

         1) the substrate lipid acid that participates in reaction is the high boiling longer chain fatty acid of the saturated short chain fatty acid of carbon chain lengths C2~

    C4 and C12;

    A, esterification;

    substrate alkyd is added by volumetric molar concentrations such as 0.4~

    3mol/L in the reaction vessel contain heptane after the stirring and dissolving, begin reaction after adding the crude fat enzyme of 18000U/L again;

    Before lipase adds reaction system, earlier lipase is stirred 15min in octane-iso, the lipase that suction filtration is removed after octane-iso obtains handling is used for above-mentioned reaction system;

    Conversion reaction is 30-50 ℃

    of reaction 12~

    48h in enclosed system, makes purpose product citronellyl acrylate, and productive rate reaches 95%;

    B, product separate;

    in the organic solvent reaction system, with lipase and reaction solution centrifugation, supernatant evaporates the organic phase reaction media through underpressure distillation, obtains the product citronellyl acrylate;

    After centrifugation, lower floor'"'"'s solid is handled through octane-iso thorough washing after drying, reclaims catalyzer zhizopchin lipase, recyclable recycling;

    Adopt the solvent-free reaction system when or

         2) the substrate lipid acid that participates in reaction is the saturated medium chain fatty acid of carbon chain lengths C5~

    C10;

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