High titer recombinant influenza viruses for vaccines

High titer recombinant influenza viruses for vaccines

  • CN 101,472,941 A
  • Filed: 03/29/2007
  • Published: 07/01/2009
  • Est. Priority Date: 03/31/2006
  • Status: Active Grant
First Claim
Patent Images

1. composition, the multiple influenza vectors that it comprises 7:

  • 1 reprovision body comprisesA) comprise the carrier of the promotor that is operably connected with the influenza virus PA cDNA that is connected to transcription termination sequence, the carrier that comprises the promotor that is operably connected with the influenza virus PB1 cDNA that is connected to transcription termination sequence, the carrier that comprises the promotor that is operably connected with the influenza virus PB2 cDNA that is connected to transcription termination sequence, the carrier that comprises the promotor that is operably connected with the influenza virus HA cDNA that is connected to transcription termination sequence, the carrier that comprises the promotor that is operably connected with the influenza virus NP cDNA that is connected to transcription termination sequence, the carrier that comprises the promotor that is operably connected with the influenza virus NA cDNA that is connected to transcription termination sequence, the carrier that comprises the promotor that is operably connected with the influenza virus M cDNA that is connected to transcription termination sequence, and the carrier that comprises the promotor that is operably connected with the influenza virus NS cDNA that is connected to transcription termination sequence, PB1 wherein, PB2, PA, NP, NA, the cDNA of M and NS has leisure that the PB1 that copies to one or more influenza viruses of high titre in embryo egg or the mdck cell is arranged, PB2, PA, NP, NA, the sequence of M and NS, and the cDNA of wherein said HA has the sequence of allos HA;

    AndB) comprise the carrier of the promotor that the dna fragmentation with encoding influenza virus PA can be operatively connected, the carrier that comprises the promotor that the dna fragmentation with encoding influenza virus PB1 can be operatively connected, the carrier that comprises the promotor that the dna fragmentation with encoding influenza virus PB2 can be operatively connected, and the carrier that comprises the promotor that the dna fragmentation with encoding influenza virus NP can be operatively connected, the carrier of the promotor that the optional dna fragmentation that comprises with encoding influenza virus HA can be operatively connected, the carrier that comprises the promotor that the dna fragmentation with encoding influenza virus NA can be operatively connected, the carrier that comprises the promotor that the dna fragmentation with encoding influenza virus M1 can be operatively connected, the carrier that comprises the promotor that the dna fragmentation with encoding influenza virus M2 can be operatively connected, or comprise the carrier of the promotor that the dna fragmentation with encoding influenza virus NS2 can be operatively connected.

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