Production of sialylated N-glycans in lower eukaryotes

Production of sialylated N-glycans in lower eukaryotes

  • CN 101,484,585 A
  • Filed: 05/04/2007
  • Published: 07/15/2009
  • Est. Priority Date: 05/05/2006
  • Status: Active Application
First Claim
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1. one kind is used for producing the proteic method of reorganization saliva acidizing sugar at host cell, and described host cell has carried out selecting or be engineered, contains Gal with generation (1-4)GlcNAc (1-4)Man 3GlcNAc 2The glycoprotein of sugar shape, said method comprising the steps of:

  • the nucleic acid that coding is had the active enzyme of sialic acid based transferase imports in the host cell, described enzyme contains sialic acid based transferase catalyst structure domain and cell-targeting signal peptide, with the Secretory Pathway with sialic acid based transferase catalyst structure domain target host cell;

    Wherein, when recombinant glycoprotein passes through the Secretory Pathway of host cell, produce and contain NANA (1-4)Gal (1-4)GlcNAc (1-4)Man 3GlcNA 2The recombinant glycoprotein of sugar shape.

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