Protein production using eukaryotic cell lines

Protein production using eukaryotic cell lines

  • CN 101,511,994 A
  • Filed: 05/22/2007
  • Published: 08/19/2009
  • Est. Priority Date: 05/22/2006
  • Status: Active Application
First Claim
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1. site-specific integration targeting vector, described carrier comprises:

  • (a) the first carrier recombination site, it is recombinated with the genome recombination site in the presence of the first unidirectional site-specific recombinase;

    (b) the second carrier recombination site, it is recombinated with the donor recombination site in the presence of the second unidirectional site-specific recombinase that is different from the described first unidirectional site-specific recombinase;

    (c) first part of first selected marker adjacent with 3 '"'"' end of the described second carrier recombination site;

    With(d) be different from second selected marker of described first selected marker.

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