Metabolic engineering of arabinose- fermenting yeast cells

Metabolic engineering of arabinose- fermenting yeast cells

  • CN 101,522,886 A
  • Filed: 10/01/2007
  • Published: 09/02/2009
  • Est. Priority Date: 10/02/2006
  • Status: Active Application
First Claim
Patent Images

1. can express the eukaryotic cell of following nucleotide sequence, wherein the expression of these nucleotide sequences is given that cell uses L-arabinose and/or L-arabinose is converted into L-ribulose and/or X 5P and/or is converted into the ability of desired fermentation product:

  • (a) nucleotide sequence of coding Arabinose isomerase (araA), wherein said nucleotide sequence is selected from the group of being made up of following;

    I. the encode nucleotide sequence of araA, described araA comprise the aminoacid sequence that the aminoacid sequence with SEQ ID NO;

    1 has at least 55% sequence identity,Ii. nucleotide sequence, it comprises the nucleotide sequence that the nucleotide sequence with SEQ ID NO;

    2 has at least 60% sequence identity,Iii. nucleotide sequence, its complementary strand with (i) or the making nucleic acid molecular hybridization of sequence (ii),Iv. nucleotide sequence, its sequence is because the degeneracy of genetic codon and different with the sequence of (iii) nucleic acid molecule;

    (b) nucleotide sequence of coding L-ribulokinase (araB), wherein said nucleotide sequence is selected from the group of being made up of following;

    I. the encode nucleotide sequence of araB, described araB comprise the aminoacid sequence that the aminoacid sequence with SEQ ID NO;

    3 has at least 20% sequence identity,Ii. nucleotide sequence, it comprises the nucleotide sequence that the nucleotide sequence with SEQ ID NO;

    4 has at least 50% sequence identity,Iii. nucleotide sequence, its complementary strand with (i) or the making nucleic acid molecular hybridization of sequence (ii),Iv. nucleotide sequence, its sequence is because the degeneracy of genetic codon and different with the sequence of (iii) nucleic acid molecule;

    (c) nucleotide sequence of coding L-ribulose-5-P-4-epimerase (araD), wherein said nucleotide sequence is selected from the group of being made up of following;

    I. the encode nucleotide sequence of araD, described araD comprise the aminoacid sequence that the aminoacid sequence with SEQ ID NO;

    5 has at least 60% sequence identity,Ii. nucleotide sequence, it comprises the nucleotide sequence that the nucleotide sequence with SEQ ID NO;

    6 has at least 60% sequence identity,Iii. nucleotide sequence, its complementary strand with (i) or the making nucleic acid molecular hybridization of sequence (ii),Iv. nucleotide sequence, its sequence is because the degeneracy of genetic codon and different with the sequence of (iii) nucleic acid molecule.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×