Heterozygosis for vertebrate cells suppresses tRNA

Heterozygosis for vertebrate cells suppresses tRNA

  • CN 101,541,955 B
  • Filed: 09/07/2007
  • Issued: 09/28/2016
  • Est. Priority Date: 09/08/2006
  • Status: Active Grant
First Claim
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1. non-human vertebrate'"'"'s cell, it comprises colibacillary tyrosyl-tRNA synthetase, by SEQ ID NO:

  • 88Coded orthogonal tRNA, selected from p-acetyl-phenylalanine and p-azido-phenylalanine alpha-non-natural amino acid withAnd comprising the nucleic acid of the polynucleotide encoding paid close attention to polypeptide, wherein said polynucleotide comprises to be known by described orthogonal tRNAOther selectivity codon, described non-human vertebrate'"'"'s cell is CHO K1 cell, described colibacillary tyrosyl tRNASynzyme preferentially utilizes described alpha-non-natural amino acid by described orthogonal tRNA/aminoacyl and described in described CHO K1 cellCHO K1 cell produces the polypeptide paid close attention in the presence of alpha-non-natural amino acid.

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