Method for modifying insect resistance of plants by utilizing rnai technique

Method for modifying insect resistance of plants by utilizing rnai technique

  • CN 101,548,017 B
  • Filed: 12/04/2007
  • Issued: 07/01/2015
  • Est. Priority Date: 12/04/2006
  • Status: Active Grant
First Claim
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1. improve a method for plant resistance to insect, it is characterized in that, described method comprises step:

  • Proceed in vegetable cell, tissue or organ by the construction of expressing insect genes dsRNA, the construction of described expression insect genes dsRNA is double-strand, and its normal chain or minus strand contain with following formula I structure;

    Seq forward-X-Seq oppositelyformula I In formula,Seq forwardfor forward sequence or the fragment of insect genes, the length of wherein said fragment is at least 50nt;

    Seq oppositelyfor following Seq forwardcomplementary sequence or fragment, the length of wherein said fragment is at least 50nt;

    X is for being positioned at Seq forwardand Seq oppositelybetween intervening sequence, and described intervening sequence and Seq forwardand Seq oppositelynot complementary;

    Described insect genes is the gene of coding GIP albumen, and the aminoacid sequence of this GIP albumen is as shown in SEQ ID NO;

    2.

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