Method for testing chromosome 22q11.2 microdeletion and microduplication

Method for testing chromosome 22q11.2 microdeletion and microduplication

  • CN 101,555,528 A
  • Filed: 03/09/2009
  • Published: 10/14/2009
  • Est. Priority Date: 09/28/2008
  • Status: Active Grant
First Claim
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1. , micro-deleted, the little multiple measuring method in a kind of chromosome 22 q11.2 district is characterized in that this method comprises the following steps:

  • A. extracting genomic dna;

    B. multiple fluorescence quantitative PCR composite amplification;

    comprised 5 STR mark;

    D22S873 in the composite amplification system, 22D_5_1,22D_4_5,22D_4_4,22D_4_3 and a confidential reference items G6PDH;

    Carry out pcr amplification;

    C. get the pcr amplification product sex change and make double-stranded product be converted into the strand that can be capillary electrophoresis analysis, adopt capillary electrophoresis that above-mentioned denatured products is carried out product length and product component analysis;

    D. interpretation of result;

    1) according to following formula calculated amount ratio;

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