Methods for selecting improved strains

Methods for selecting improved strains

  • CN 101,578,364 A
  • Filed: 01/08/2008
  • Published: 11/11/2009
  • Est. Priority Date: 01/10/2007
  • Status: Active Application
First Claim
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1. obtain the method for the filamentous fungal strains of generation purpose enzyme, described method comprises:

  • (a) parental cell of filamentous fungal strains is gone up different population of test with material contact that produces genetic diversity thereby generation heredity;

    With(b) select step, it is included in and contains in the substratum of Mierocrystalline cellulose as unique source of carbon and energy, and is suppressing to cultivate described population of test under the temperature that described parental cell produces described enzyme;

    With(c) cell of separation improving from described population of test, the level of the described enzyme of its generation are that (i) is higher than level that parental cell produces or (ii) under the temperature that is higher than the suitableeest production temperature of parental cell, is equal to or higher than the level that parental cell produces.

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