The reaction buffer that is used for microarray

The reaction buffer that is used for microarray

  • CN 101,595,231 A
  • Filed: 12/29/2006
  • Published: 12/02/2009
  • Est. Priority Date: 12/29/2006
  • Status: Active Application
First Claim
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1. , is used for the buffer composition of nucleic acid amplification and hybridization integrated reaction, comprises:

  • the salt of about 50-200mM;

    The Tris-HCl of about 10-30mM;

    The water-soluble magnesium salt of about 2-10mM;

    The tensio-active agent of about 0.05-1.5%;

    One or more primers of about 50-300nM;

    One or more dNTPs of about 20-150 μ

    M;

    The glycerine of about 5-15%;

    The methane amide of about 0.5-1.5%;

    With polysaccharase at least about 5 unit/ml.

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