The production method of L-arabinose and D-xylose pref

The production method of L-arabinose and D-xylose pref

  • CN 101,665,523 B
  • Filed: 09/30/2009
  • Issued: 09/30/2015
  • Est. Priority Date: 09/30/2009
  • Status: Active Grant
First Claim
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1. a production method for L-arabinose and xylose pref, with the hydrolyzed solution of agriculture and forestry organic waste material, xylose mother liquid and/or the production waste liquid containing five-carbon sugar for raw material, at least comprises the following steps:

  • A described Feedstock treating is the total reducing sugar feed liquid at least comprising wood sugar, xylan, L-arabinose, glucose and semi-lactosi by the pre-treatment of () feed liquid;

    B () chromatographic separation, is separated into pectinose fraction feed liquid and xylose fractions feed liquid by described total reducing sugar feed liquid;

    C () comprises the following steps (c1) and (c2),(c1) except fermentation inhibitor, described fermentation inhibitor is the impurity and the miscellaneous bacteria that fermentation are had to suppression;

    (c2) ferment removal of impurities sugar, and described assorted sugar at least comprises glucose and semi-lactosi;

    This two step or be placed in step (b) chromatographic separation before described total reducing sugar feed liquid processed or is placed in the chromatographic separation of step (b) after described pectinose fraction feed liquid and xylose fractions feed liquid carried out respectively to the process of following two steps;

    D () is finally carried out aftertreatment respectively to described pectinose fraction feed liquid and xylose fractions feed liquid and is obtained L-arabinose and xylose pref;

    The feed liquid pre-treatment of the hydrolyzed solution of described agriculture and forestry organic waste material comprises the following steps;

    1. clean;

    silt and the chip of removing described agriculture and forestry organic waste material surface;

    2. dilute acid pretreatment;

    soak described agriculture and forestry organic waste material to remove impurity with diluted acid, described dilute acid concentration is at 0.05 ~ 0.15% wt, and temperature is 110 ~ 130 DEG C, and the treatment time is 1 ~ 3 hour;

    Described diluted acid is selected from the mixed solution of acid two or more in sulfuric acid, hydrochloric acid, phosphoric acid, acetic acid, nitric acid or these acid;

    3. be hydrolyzed;

    add the dense acid solution at 0.5 ~ 1% wt of acid, temperature 128 ~ 132 DEG C insulation 2.5 hours;

    Described acid solution is selected from the mixed solution of acid two or more in sulfuric acid, hydrochloric acid, phosphoric acid, acetic acid, nitric acid or these acid;

    4. neutralize;

    first liquid glucose is heated to 80 ~ 82 DEG C, then adds calcium carbonate powders, until pH rises to 3.3 ~ 3.6, when mineral acid is at 0.09 ~ 0.12% wt, then add gac;

    5. first time decolouring;

    first liquid glucose is cooled to 50 ~ 52 DEG C, then adds gac and stirs until transparence reaches 60 ~ 76%;

    6. first time desalination;

    adopt ion-exchange, electrodialysis or EDI electricity desalination method the ash content, salt, organic acid and the mineral acid that contain in first time destainer to be removed;

    7. first time evaporation;

    adopt triple effect or quadruple effect falling-film evaporator that sugar concentration is brought up to 26.0 ~ 28.0%;

    8. second time decolouring;

    add gac and stir;

    9. second time desalination;

    adopt ion-exchange, electrodialysis or EDI electricity desalination method the ash content contained in second time destainer, salt, organic acid and mineral acid to be removed;

    The pre-treatment of described xylose mother liquid and/or the described waste liquid containing five-carbon sugar comprises;

    1. filter;

    first adopt mechanical filter equipment, adopt membrane filter plant to filter again;

    Described mechanical filter equipment is selected from plate-and-frame filter press, bag type filtering machine, horizontal filtering machine, microfroc filter and filtering centrifuge;

    Described membrane filter plant is selected from ceramic membrane, metallic membrane, organic rolled film and tubular membrane, retains and is of a size of 100 ~ 5000 g/mol;

    2. desalination;

    adopt the mode of ion-exchange or adopt electrodialysis, EDI electricity desalination method;

    Described fermentation inhibitor in step (c1) comprises impurity and miscellaneous bacteria, impurity wherein comprises the band coloring matter of phenyl ring, macromolecular pigment, macromolecular compound, heavy metal ion, muriate, colloid and polymkeric substance, and miscellaneous bacteria wherein comprises yeast in nature air and bacterium;

    Described step (c1) except fermentation inhibitor further comprising the steps;

    1. for the charged ion in described impurity, muriate and colloid, the method for electrodeionization or ion-exchange is adopted to be removed;

    2. for the coloring matter in described impurity, macromolecular compound, polymkeric substance and miscellaneous bacteria, membrane filtration or the real method disappeared is adopted to be removed;

    The method that described reality disappears carries out sterilising treatment for feed liquid is heated to 60 ~ 120 DEG C,The byproduct that step is produced of the fermentation removal of impurities sugar of described step (c2) comprising;

    ethanol, D-ribose and/or citric acid, and described step (c2) comprises one of them of following methods;

    1. shikimic acid defective type subtilis is used to generate D-ribose at aerobic condition bottom fermentation glucose, semi-lactosi;

    2. fermentation of Aspergillus niger glucose, semi-lactosi is used to generate citric acid.

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