Method for qualification of first specific mRNA with specific sequence

Method for qualification of first specific mRNA with specific sequence

  • CN 101,696,448 A
  • Filed: 10/29/2004
  • Published: 04/21/2010
  • Est. Priority Date: 10/30/2003
  • Status: Active Application
First Claim
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1. one kind is carried out quantitative methods to first specific mrna that contains from the sample particular sequence, comprising:

  • A) use second specific mrna of known quantity to mix the described sample of mark;

    B) the poly AmRNA in the purification of samples;

    C) prepare cDNA by the mRNA in the sample;

    D) corresponding to each quantitative cDNA of first specific mrna in the sample and second specific mrna;

    E) determine the recovery percent of second specific mrna;

    AndF) recovery percent of use second specific mrna is determined the original bulk of first specific mrna.

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