Method for detecting bar-transgenic sugarcane and appliance

Method for detecting bar-transgenic sugarcane and appliance

  • CN 101,698,883 B
  • Filed: 10/29/2009
  • Issued: 01/28/2015
  • Est. Priority Date: 10/29/2009
  • Status: Active Grant
First Claim
Patent Images

1. a detection turns barthe method of gene sugarcane, is characterized in that comprising the following steps:

  • (1) the wild-type Sugarcane Leaves that growth site is consistent is chosen, be cut into the segment that length is 2 ~ 5cm, be immersed in the phosphinothricin solutions of different concns gradient of 5ml respectively, then be placed in growth cabinet and carry out cultivation 3 ~ 5 days observationss, determine the minimum concentration of the phosphinothricin solutions that can injure wild-type Sugarcane Leaves, the concentration of the phosphinothricin solutions causing wild-type Sugarcane Leaves just all to turn yellow is minimum concentration;

    (2) Sugarcane Leaves to be detected is got, be cut into the segment that length is 2 ~ 5cm, get the identical wild-type Sugarcane Leaves of tender degree old with Sugarcane Leaves to be measured in contrast simultaneously, be cut into 2 ~ 5cm segment equally, be placed in the minimum concentration that 5ml can injure the phosphinothricin solutions of wild-type Sugarcane Leaves respectively, in growth cabinet, cultivate 3 ~ 5 days with the culture condition identical with step (1);

    Then by comparing with wild-type Sugarcane Leaves in contrast, what blade maintenance was green is antiweed transgenic sugarcane.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×